N1891
p-Nitrophenyl phosphate
SIGMAFAST™, chromogenic, tablet
Sinónimos:
pNPP tablets
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About This Item
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Nombre del producto
SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
Quality Level
form
tablet
solubility
deionized water: soluble 5 mL
storage temp.
−20°C
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Application
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
- to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)
- to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay
- as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)
- as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin
Reconstitution
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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