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Merck

KCQS03

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix

iQ, with fluorescein for Bio-Rad systems

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

form

liquid

usage

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

feature

dNTPs included
hotstart

storage condition

protect from light

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

compatibility

for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5

detection method

SYBR® Green

shipped in

dry ice

storage temp.

−20°C

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General description

KiCqStart SYBR Green qPCR ReadyMix es una mezcla maestra lista para usar concentrado X2 que contiene todos los componentes, excepto los cebadores y la plantilla, para la PCR cuantitativa (qPCR) en tiempo real Esta combinación única de tampón patentado, estabilizadores y ADN polimerasa Taq de inicio en caliente proporciona la eficiencia, sensibilidad y especificidad máximas de la PCR y una señal fluorescente robusta cuando se utilizan protocolos de ciclado rápidos o convencionales con SYBR Green qPCR.

Es crucial una amplificación muy específica para conseguir una qPCR satisfactoria con tecnología de colorante SYBR Green I porque este colorante se une a, y detecta, cualquier dsDNA generado durante la amplificación. La ADN polimerasa Taq de inicio en caliente es inactivada por anticuerpo antes de la etapa inicial de desnaturalización de la PCR.

Application

Aplicaciones de la PCR:
  • Expresión génica
  • Cuantificación del ADN
  • CHiP
KiCqStart® SYBR® Green qPCR ReadyMix has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.

Features and Benefits

  • Resultados del análisis en tan sólo 33 minutos
  • Resultados de la PCR en tiempo real muy eficaces y sensibles
  • Se requiere poca o ninguna optimización

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Other Notes

Condiciones de conservación:
La mezcla KiCqStart SYBR Green qPCR ReadyMix es estable durante 1 año cuando se conserva en un refrigerador a una temperatura constante de -20°C, protegida de la luz. Para su conveniencia, puede conservarse sin congelar entre +2 y +8°C durante un máximo de 6 meses. Después de descongelar, mezcle bien antes de usar. No se recomienda congelar y descongelar repetidamente el producto. Sin embargo, se demostró que el producto no perdía rendimiento después de 20 ciclos de congelación-descongelación o 2 meses a +20°C.

Legal Information

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificados de análisis (COA)

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Himanshu Kumar Khuntia et al.
SN applied sciences, 2(8), 1320-1320 (2020-08-25)
This research aims to determine the presence of antibiotic-resistant genes (ARG) in anaerobic biofilm reactors (ABR) fed with household chemical products (HCP) such as laundry detergents and handwash without any influx of antibiotics. The ABR comprised a three-chamber design with
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 43(12), 1161-1171 (2013-10-26)
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 48, 8-16 (2014-03-04)
The yellow-fever mosquito Aedes aegypti is a major vector of human diseases, such as dengue, yellow fever, chikungunya and West Nile viruses. Chemoreceptor organs on the labella and tarsi are involved in human host evaluation and thus serve as potential
Glade Dlott et al.
Journal of microbiological methods, 115, 112-120 (2015-06-10)
We tested a method of estimating the activity of detectable individual bacterial and archaeal OTUs within a community by calculating ratios of absolute 16S rRNA to rDNA copy numbers. We investigated phylogenetically coherent patterns of activity among soil prokaryotes in
Stephanie Yarwood et al.
Microbial ecology, 69(2), 383-394 (2014-11-06)
The process of pedogenesis and the development of biological communities during primary succession begin on recently exposed mineral surfaces. Following 30 years of surface exposure of reclaimed surface mining sites (Appalachian Mountains, USA), it was hypothesized that microbial communities would

Artículos

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Protocolos

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.

Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specific experimental needs.

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency is compared over a wide and narrow dynamic range of cDNA concentrations.

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Contenido relacionado

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

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