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HGECKO2G
Gecko2 Human Whole Genome CRISPR Pool, gRNA Only Lenti Particles (Gecko2 vector)
About This Item
Productos recomendados
packaging
pkg of 8x25 μL (vials)
Quality Level
concentration
5x108 VP/ml (via p24 assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
Categorías relacionadas
1 of 4
Este artículo | 143480 | M5852 | A41208 |
---|---|---|---|
Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
refractive index n20/D 1.642 (lit.) | refractive index n20/D 1.656 (lit.) | refractive index - | refractive index - |
form liquid | form liquid | form - | form crystals |
mp 16-20 °C (lit.) | mp - | mp 127-130 °C (lit.) | mp 103-107 °C (lit.) |
density 1.17 g/mL at 25 °C (lit.) | density 1.179 g/mL at 25 °C (lit.) | density - | density - |
General description
Application
Features and Benefits
- Use CRISPR nucleases to knockout protein-coding genes to assess their function
- Efficiently screen the whole human genome (16,000+ genes) at the bench-top without robotics or specialized equipment
- Numerous built-in enrichment and depletion controls allow researchers to confidently gauge the success of their pooled screening experiments
- Lentiviral CRISPRs can infect a broad variety of mammalian cells by transducing a single guide RNA (sgRNA) to a Cas9-expressing human cell line to facilitate gene knockout for screening applications.
- Use the dual vector system for the human GeCKO version 2 libraries for human cell lines that have Cas9 already integrated into the genome.
- Use puromycin gRNA selection after transduction.
Preparation Note
Other Notes
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts.
Protocolos
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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