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GE27-5330-01

Microspin™ G-50 Columns

Cytiva 27-5330-01, pack of 50 columns

Sinónimos:

DNA Purification Columns, G-50 Columns, G-50 Microspin Columns

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

material

resin (Sephadex G-50 DNA Grade)

sterility

non-sterile

packaging

pack of 50 columns

manufacturer/tradename

Cytiva 27-5330-01

working volume

12—50 μL

particle size

50-150 μm (dry)

pore size

30 kDa MWCO

Categorías relacionadas

General description

MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.

Features and Benefits

  • For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography.
  • Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
  • Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
  • Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μ) following heat denaturation and phenol extraction.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Storage Class

12 - Non Combustible Liquids

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Supelco

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