Saltar al contenido
Merck
Todas las fotos(1)

Documentos

GE27-5325-01

Microspin G-25 Columns

Cytiva 27-5325-01

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 27-5325-01

packaging

pkg of 50 columns

particle size

20-80 μm (dry)

working range

2-13

Categorías relacionadas

General description

MicroSpin G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

MicroSpin G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.

Features and Benefits

  • For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
  • Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
  • Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
  • Ready to use: Requires less than 4 min from sample application to collection of purified product.
  • Tested in nickase, single and double-stranded exonuclease and RNase assays.
  • Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.

Storage and Stability

Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Storage Class

12 - Non Combustible Liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Artículos

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page shows how to prepare samples for purification with affinity chromatography.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico