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Merck

G1777

Sigma-Aldrich

Glial Cell Line-derived Neurotrophic Factor human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE)

Sinónimos:

ATF, GDNF

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About This Item

Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

human

Nivel de calidad

recombinante

expressed in E. coli

Ensayo

≥98% (SDS-PAGE)

Formulario

lyophilized powder

mol peso

~30 kDa

envase

pkg of 10 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

color

white

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

human ... GDNF(2668)

Descripción general

Glial cell line-derived neurotrophic factor (GDNF) human gene is located on human chromosome 5p13.2.

Aplicación

Glial cell line-derived neurotrophic factor human (GDNF) has been used as a component in the neurobasal medium for neural differentiation. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).

Acciones bioquímicas o fisiológicas

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.
Glial cell line-derived neurotrophic factor human (GDNF) acts as a morphogen in kidney development and modulates spermatogonial differentiation. Mutations in this gene may be associated with Hirschsprung′s disease, Tourette syndrome (TS) and attention deficit/ hyperactivity disorder (ADHD). It is used to treat Parkinson′s disease.

Forma física

Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.

Nota de análisis

The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Visite la Librería de documentos

Novel functions and signalling pathways for GDNF
Sariola H and Saarma M
Journal of Cell Science, 116(19), 3855-3862 (2003)
GDNF gene is associated with tourette syndrome in a family study
Huertas-Fernandez I, et al.
Movement Disorders, 30(8), 1115-1120 (2015)
Jacob L Roam et al.
Biomaterials, 35(24), 6473-6481 (2014-05-13)
Introduction of spatial patterning of proteins, while retaining activity and releasability, is critical for the field of regenerative medicine. Reversible binding to heparin, which many biological molecules exhibit, is one potential pathway to achieve this goal. We have covalently bound
Generation of Mouse Spermatogonial Stem-Cell-Colonies in A Non-Adherent Culture
Azizi H, et al.
Cell Journal, 19(2), 238-238 (2017)
Gain at chromosomal region 5p15. 33, containing TERT, is the most frequent genetic event in early stages of non-small cell lung cancer
Kang JU, et al.
Cancer Genetics and Cytogenetics, 182(1), 1-11 (2008)

Artículos

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