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F4165

Sigma-Aldrich

Ficin from fig tree latex

powder, ≥0.1 unit/mg solid

Sinónimos:

Debricin, Ficain, higueroxyl delabarre

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About This Item

Número de CAS:
9001-33-6
Comisión internacional de enzimas:
3.4.22.3 (BRENDA, IUBMB)
EC Number:
232-599-1
MDL number:
MFCD00081597
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

fig tree (latex)

form

powder

specific activity

≥0.1 unit/mg solid

mol wt

23.8 kDa

storage temp.

−20°C

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General description

Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.

Unit Definition

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.

inhibitor

Referencia del producto
Descripción
Precios

E3132

E-64, protease inhibitor

substrate

Referencia del producto
Descripción
Precios

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Pepsin lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Roche

10108057001

Pepsin

Robertson D Davenport et al.
Transfusion, 44(6), 849-852 (2004-05-26)
Cefotetan can cause severe immune hemolytic anemia that may persist long after the drug is discontinued. To study the binding of cefotetan to RBCs, patients who received cefotetan were followed and tested for the presence of antibody to cefotetan. Patients
V B Reddy et al.
The British journal of dermatology, 163(3), 532-535 (2010-05-25)
Bromelain, ficin and papain are cysteine proteases from plants that produce itch upon injection into skin. Their mechanism of action has not been considered previously. To determine the mechanism by which these proteases function. The ability of these proteases to
A Saito et al.
Biochemistry and molecular biology international, 46(1), 27-34 (1998-10-24)
Amino acid sequencing of the ficin-derived C-terminal fragments of alpha-1-antiproteinase (also called alpha-1-antitrypsin or alpha-1-proteinase inhibitor) prepared from rabbit plasma revealed the presence of the E isoform, which had been confirmed in the cDNA library in addition to the F
Sadami Ohtsubo et al.
Journal of agricultural and food chemistry, 53(13), 5218-5224 (2005-06-23)
On the basis of cDNA sequences, we found that the calli of rice encodes an amino acid sequence that shares 56% and 89% identity, respectively, with oryzacystatin-I and oryzacystatin-II. This sequence differs from that of oryzacystatin-II in the N-terminal region
J D Reid et al.
The Biochemical journal, 357(Pt 2), 343-352 (2001-07-06)
The possibility of a slow post-acylation conformational change during catalysis by cysteine proteinases was investigated by using a new chromogenic substrate, N-acetyl-Phe-Gly methyl thionoester, four natural variants (papain, caricain, actinidin and ficin), and stopped-flow spectral analysis to monitor the pre-steady
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Protocolos

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

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