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10108057001

Roche

Pepsin

lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Sinónimos:

pepsin

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About This Item

Comisión internacional de enzimas:
3.4.23.1 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

pig gastric mucosa

Quality Level

100

form

lyophilized (salt-free)

specific activity

~2500 units/mg protein

packaging

pkg of 1 g

manufacturer/tradename

Roche

optimum pH

1.8-2.2

shipped in

wet ice

storage temp.

2-8°C

General description

Pepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.

Specificity

Aspartic endopeptidase with relatively broad specificity. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1′ positions.

Application

Pepsin has been used for the digestion of liver tissue obtained from rat.

Preparation Note

Working concentration: 0.05 to 0.5 mg/ml
Storage conditions (working solution): A pepsin solution is stable at 2 to 8 °C, at least for one week, at neutral pH and under germfree conditions. Frozen aliquots of the enzyme solution are expected to be more stable.

A stock solution in 10 mM HCl (5-10 mg/ml) should be stable for at least one week at 2 to 8 °C, too. Storage light protected, tightly closed.

A short (2 minutes) incubation at 40 °C has negligible effect on the activity of lyophilized pepsin or pepsin in solution.

Analysis Note

At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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