K-BALB (K-234)

89092101

• UNSPSC Code: 41106514

Propiedades

• biological source: mouse embryo
• growth mode: Adherent
• karyotype: 2n=40
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse A31 embryo Kirsten sarcoma, transformed

Cell Line Description

Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO₂; 37°C

Other Notes

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