CCRF-SB

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Propiedades

• biological source: human blood
• packaging: tube of 5 μg 89090405-DNA-5UG; pkg of vial of cells 89090405-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian acute lymphoblastic leukaemia

Cell Line Description

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

Application

HLA profile studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.