APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Sinónimos:
Red fluorescent Annexin V apoptosis assay
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.32
Productos recomendados
usage
sufficient for 200 tests
Quality Level
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Principle
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Legal Information
Cy3 is a trademark of Cytiva
Solo componentes del kit
Referencia del producto
Descripción
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- C50416-Carboxyfluorescein diacetate 10 mgSDS
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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