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Merck

T5428

Sigma-Aldrich

Tetramethylrhodamine methyl ester perchlorate

≥95% purity, powder

Sinónimos:

TMRM

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About This Item

Fórmula lineal:
C25H25N2O3 · ClO4
Número de CAS:
Peso molecular:
500.93
Beilstein/REAXYS Number:
7162165
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Tetramethylrhodamine methyl ester perchlorate, ≥95%

Quality Level

assay

≥95%

form

powder

composition

Carbon: 57.5-62.4%

Nitrogen: 5.3-5.9%

color

dark green

solubility

methanol: 5 mg/mL, clear

εmax

>95000 at 546-552 nm in methanol

fluorescence

λem 573 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.COC(=O)c1ccccc1C2=C3C=C\C(C=C3Oc4cc(ccc24)N(C)C)=[N+](\C)C

InChI

1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1

InChI key

PFYWPQMAWCYNGW-UHFFFAOYSA-M

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General description

Tetramethylrhodamine methyl ester perchlorate (TMRM) is a membrane-permeant, cationic, fluorescent dye that can label mitochondria and mark embryonic stem cell/induced pluripotent stem cell-derived cardiomyocytes. It is useful for the isolation of mature cardiomyocytes.

Application

Tetramethylrhodamine methyl ester perchlorate (TMRM) has been used as a sensitive probe for the detection of mitochondrial membrane potential.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Journal of cell science, 132(15) (2019-07-11)
Mitochondrial remodelling is a central feature of stem cell differentiation. However, little is known about the regulatory mechanisms during these processes. Previously, we found that a pharmacological inhibitor of glycogen synthase kinase-3α and -3β, CHIR-99021, initiates human adipose stem cell

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