SCR011

B6-White Murine ES Cell Line

The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function.

• UNSPSC Code: 41106509
• eCl@ss: 32011203
• NACRES: NA.81

Propiedades

• biological source: mouse
• Quality Level: 100
• manufacturer/tradename: B6-White
• technique(s): cell culture | stem cell: suitable
• input: sample type: mouse embryonic stem cell(s)
• shipped in: liquid nitrogen

Descripción

General description

The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function. The influence of genetic background on phenotype has been shown to be an important consideration in selection of a mouse model. Furthermore, the time required to achieve congenic status, which can be 18-24 months has further stimulated demand for targeting in pure inbred strains including C57BL/6 (B6) mice.

Millipore′s B6-White Murine ES cell line is the first commercially available C57BL/6 tyrc-2J albino line that allows for rapid coat-color determination of successful chimerism in the C57BL/6 mouse strain. When B6-White ES cells are injected into C57BL/6 blastocysts, chimeric mice are easily identified by their coat color (a mix of black and white patches), whilst non-chimeric littermates are black. These cells allow for the efficient generation of gene-targeted mice in a pure C57BL/6 genetic background, thus providing more experimental flexibility. Additionally the use of these cells can lower production costs by eliminating the need to maintain an albino blast donor colony, in order to assess chimerism by coat color when targeting in the C57BL/6 strain.

For germline transmission confirmation by coat color, it is recommended that male chimeras are mated with homozygous females of the B6-white strain (strain name: C57BL/6J-Tyrc-2J/J - available from Jackson Labs, stock number 000058).

STRAIN:C57BL/6-tyr(c-2J) albino mice

GENDER: Male

KARYOTYPE: 40XY

Cell Line Description

Embryonic Stem Cells

Application

Research Category
Stem Cell Research

Physical form

Cells are supplied frozen in basal medium supplemented with 10% FCS and 10% DMSO

Storage and Stability

Place vials in the vapor phase of liquid nitrogen storage immediately upon receipt until it is convenient to proceed to subculture.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2