ES-E14TG2a

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 08021401, mouse embryo (blastocyst). Monolayer of spheroidal cells on feeder layer of PMEFs or STOs

• Sinónimos: E14TG2a Cell Line
• UNSPSC Code: 41106514

Propiedades

• biological source: mouse embryo (blastocyst)
• packaging: tube of 5 μg 08021401-DNA-5UG; pkg of vial of cells 08021401-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Descripción

Cell Line Origin

Mouse (strain 129/Ola) embryonic stem cell

Cell Line Description

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

DNA Profile

Not specified

Culture Medium

DMEM + 2 mM Glutamine + 1% NEAA + 0.1 mM 2-Mercaptoethanol + 1000 U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Fetal Bovine Serum. Renewal of medium every 2-3 days is required.

Subculture Routine

Not specified

Other Notes

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