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Ascentis® Express 160 Å C30 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Ascentis® Express 160 Å C30, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Ascentis® Express 160 Å C30, 2.7 μm HPLC Column, column L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L62

product line

Ascentis®

feature

endcapped

packaging

1 ea of

extent of labeling

4.5% Carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable

column L × I.D.

15 cm × 4.6 mm

surface area

90 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

C30 (triacontyldimethyl) bonding phase

particle size

2.7 μm

pore size

160 Å pore size

operating pH range

2-9

separation technique

reversed phase

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General description

Ascentis® Express 160 Å C30 is a high-speed, high-performance liquid chromatography column based on the wider-pore 160 Å Fused-Core® particle design to provide adequate spacing for the long-chain C30 ligands. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The endcapped triacontylsilane stationary phase of the C30 provides a stable, reversed-phase packing that can be used for hydrophobic, long-chain, structural isomers such as vitamins and lipids.

Application

UHPLC Analysis of Vitamin A and Vitamin E on Ascentis® Express C30
UHPLC/MS Analysis of Corn Oil on Ascentis® Express C30 Compared to Ascentis® Express C18
UHPLC Analysis of Vitamin K1 on Ascentis® Express C30
HPLC Analysis of Tocopherols on Ascentis® Express C30, 2.7 μm

Analysis Note

The target analytes include hydrophobic analytes, lipids, carotenoids, and fat soluable vitamins (A, D, E, K).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USCEQ001355
USCEQ001354
USCEQ001353
USCEQ001352
USCEQ001351

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Protocols

Determination of Vitamin A and E Using HPLC with UV Detection Following the Chinese National Standard (GB) Method

Following GB method for Vitamin A and Vitamin E as it is written with a 250 x 4.6 mm Ascentis® Express C30 column gives baseline resolution between the critical pair (gamma-, and beta-tocopherol; peaks 3 and 4).

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