I4777
Interferon-γ from mouse
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
IFN-γ
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About This Item
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biological source
mouse
Quality Level
recombinant
expressed in E. coli
Assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
protein 15.6 kDa
packaging
pkg of 0.1 mg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
solubility
water: soluble 1.000 mL, clear, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Ifng(15978)
Application
Interferon-γ from mouse has been used:
- for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c+ (CD11 antigen-like family member C) lung macrophages
- for stimulation of murine macrophages to induce the production of nitrite and nitrate
- to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation
Biochem/physiol Actions
Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.
Physical form
Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.
Analysis Note
The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Sergio Arias-Negrete et al.
Analytical biochemistry, 328(1), 14-21 (2004-04-15)
Preparation of a nitrate reductase lysate of Escherichia coli MC1061 to measure nitrate and nitrite in biologic fluids is described. To obtain the crude bacterial lysate containing nitrate reductase activity, E. coli MC1061 was subjected to 16-20 freeze-thawing cycles, from
S N Vogel et al.
Infection and immunity, 38(2), 681-685 (1982-11-01)
Silica has been used for many years as an agent which selectively alters macrophage functions and, as such, has been used to assess the role of macrophages in the immune response to a variety of microbial and chemically defined agents.
P W Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(19), 5842-5846 (1983-10-01)
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of
Marta Pulido-Salgado et al.
Scientific reports, 8(1), 16096-16096 (2018-11-02)
Microglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with
Andre Heinen et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 27(1), 46-58 (2018-12-12)
Insulin-like growth factor 1 (IGF1) is an anabolic hormone that controls the growth and metabolism of many cell types. However, IGF1 also mediates cardio-protective effects after acute myocardial infarction (AMI), but the underlying mechanisms and cellular targets are not fully
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