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Key Documents

IF002

Sigma-Aldrich

Interferon-γ Protein, Recombinant human

The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.

Synonym(s):

Human Interferon, Human Interferon-γ, Recombinant Interferon-γ

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

biological source

human

Quality Level

100

Assay

>98% (SDS-PAGE and HPLC)

specific activity

≥2 x 10(E7) U/mg

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)

input

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)

NCBI accession no.

NM_000619.2

UniProt accession no.

P01579

shipped in

dry ice

Gene Information

human ... IFNG(3458)

General description

Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Product Source: Human, expressed in E.coli

Application

For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.

Linkage

Replaces: IF3

Physical form

Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.

Storage and Stability

The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.

Analysis Note

Specific Activity: Human IFN-gamma is fully biologically active when compared to standards.The ED(50) as determined in a viral resistance assay is ≤ 0.05 ng/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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