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G9768

Sigma-Aldrich

Guanosine 5′-triphosphate–Agarose

aqueous glycerol suspension

Synonym(s):

5′-GTP agarose

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About This Item

MDL number:
MFCD00171655
UNSPSC Code:
41106500
NACRES:
NA.56

biological source

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

Quality Level

200

form

aqueous glycerol suspension

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

ribose hydroxyls

matrix spacer

11 atoms (adipic acid dihydrazide)

storage temp.

−20°C

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Application

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physical form

Guanosine 5′-triphosphate–Agarose has been used:
  • in chromatographic column for the purification of a translocation stimulating factor
  • in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
  • in GTP-Binding assay
  • for the depletion of GTP binding proteins in cytosol of rat liver
Suspension in 50% glycerol containing 0.25 M NaCl

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC0529
SLBT4518
SLBQ8686V
SLBP5064V
SLBM8572V

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P E Glaser et al.
Biochemistry, 34(38), 12193-12203 (1995-09-26)
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Mamais A, et al.
The Journal of Biological Chemistry, 289, 21386-21400 (2014)
Reiaz Ul Rehman et al.
Plant & cell physiology, 49(5), 751-766 (2008-04-04)
The regulatory functions of Rab proteins in membrane trafficking lie in their ability to perform as molecular switches that oscillate between a GTP- and a GDP-bound conformation. The role of tomato LeRab11a in secretion was analyzed in tobacco protoplasts. Green
M Bomsel et al.
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Somatic mutations in TSC1 and TSC2 cause focal cortical dysplasia
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