E2884
Esterase from porcine liver
ammonium sulfate suspension, ≥150 units/mg protein (biuret)
Synonym(s):
Carboxyl esterase, Carboxylic-ester hydrolase, PLE
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
Porcine liver
form
ammonium sulfate suspension
specific activity
≥150 units/mg protein (biuret)
mol wt
168 kDa
storage temp.
2-8°C
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General description
Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.
Application
Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.
The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Biochem/physiol Actions
Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.
Unit Definition
One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4, pH 8
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Lipolysis and Metabolism of Fatty Acids in Cheese
Cheese, 423-444 (2017)
A New Screen for Combinatorial Catalysis; On-Bead Testing in Agarose Gel Financial support for this work was provided by the University of Bristol, the EPSRC (studentship to T.W.M.) and the EC (TMR and Human Potential programs). We are grateful to Dr. John Crosby and Dr. Russell Cox for advice on enzyme chemistry and the handling of electrophoresis gels.
Michael Müller et al.
Angewandte Chemie (International ed. in English), 40(20), 3813-3815 (2001-10-23)
Karl Vollmann et al.
Molecules (Basel, Switzerland), 13(2), 348-359 (2008-02-29)
The compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4) was synthesized as an amino acid ester prodrug of the adenosine A2A receptor antagonist MSX-2. It was found to be stable in artificial gastric acid, but readily cleaved by pig liver esterase.
Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells
Watkins RW, et al.
Organic & Biomolecular Chemistry, 7(19), 3969-3975 (2019)
F Dorandeu et al.
Toxicology, 248(2-3), 151-157 (2008-05-03)
Organophosphorus chemical warfare agents (nerve agents) are to be feared in military operations as well as in terrorist attacks. Among them, VX (O-ethyl-S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is a low volatility liquid that represents a percutaneous as well as an inhalation hazard if
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protocols
Enzymatic Assay of Esterase
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