N8130

4-Nitrophenyl acetate

esterase substrate

• Synonym(s): Acetic acid 4-nitrophenyl ester
• Linear Formula: CH₃CO₂C₆H₄NO₂
• CAS Number: 830-03-5
• Molecular Weight: 181.15
• Beilstein: 515874
• EC Number: 212-593-5
• MDL number: MFCD00007326
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897853
• NACRES: NA.32

Properties

• Quality Level: 200
• Assay: ≥98% (TLC)
• form: powder or crystals
• mp: 75-77 °C (lit.)
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C8H7NO4/c1-6(10)13-8-4-2-7(3-5-8)9(11)12/h2-5H,1H3
• InChI key: QAUUDNIGJSLPSX-UHFFFAOYSA-N
• Gene Information: human ... PON1(5444)

Description

Application

4-Nitrophenyl acetate has been used:

• to evaluate esterase activity in human embryonic kidney cell line
• in carbonic anhydrase inhibition assay
• to study its conversion to p-nitrophenol (p-NP) by the esterase activity of bovine serum albumin
• as a substrate to determine carboxylesterase (CES) activity in mouse liver cells
• to study the advantages of bioconjugation of cross-linked enzyme aggregates (CLEAs) and magnetic iron oxide nanoparticles (MIONPs) in magnetic CLEAs matrix

Substrates

Chromogenic esterase substrate.

Safety Information

• Pictograms: GHS03,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H272 - H317 - H318
• Precautionary Statements: P210 - P220 - P261 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Ox. Sol. 3 - Skin Sens. 1
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 3
• Flash Point(F): No data available
• Flash Point(C): No data available
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves