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MABN139

Sigma-Aldrich

Anti-mGluR5 Antibody, clone 11D9.1

clone 11D9.1, from mouse

Synonym(s):

Metabotropic glutamate receptor 5, mGluR5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11D9.1, monoclonal

species reactivity

human, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_000833

UniProt accession no.

P41594

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRM5(2915)

General description

Metabotropic glutamate receptor 5 (mGluR5) is a member of the G-protein coupled receptor family and functions as a receptor for glutamate. mGluR5 activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.

Immunogen

GST-tagged recombinant protein corresponding to human mGluR5.

Application

Detect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone 11D9.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected mGluR5 in normal human brain tissue.

Quality

Evaluated by Western Blotting in rat brain micropreparation tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected mGluR5 in 10 µg of rat brain micropreparation tissue lysate. The lysate was not boiled during preperation for western blotting.

Target description

~150 kDa observed. The calculated molecular weight is 130 kDa, however mGluR5 has been shown as a ~150 kDa band in western blots (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616).

Physical form

Format: Purified

Analysis Note

Control
Rat brain micropreparation tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kelly Smart et al.
Journal of psychiatry & neuroscience : JPN, 46(1), E1-E13 (2020-06-20)
Glutamate transmission is implicated in drug-induced behavioural sensitization and the associated long-lasting increases in mesolimbic output. Metabotropic glutamate type 5 (mGlu5) receptors might be particularly important, but most details are poorly understood. We first assessed in mice (n = 51

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