AB5675
Anti-Metabotropic Glutamate Receptor 5 Antibody, pain
Chemicon®, from rabbit
Synonym(s):
Metabotropic glutamate receptor 5, Metabotropic Glutamate Receptor 5 Antibody, mGluR5, Anti-mGluR5
Sign Into View Organizational & Contract Pricing
All Photos(6)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology), bovine (based on 100% sequence homology), primate (based on 100% sequence homology), horse (based on 100% sequence homology), feline (based on 100% sequence homology)
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GRM5(2915)
General description
Metabotropic glutamate receptor 5 or mGluR5 (UniProt Q3UVX5; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5, AI850523, 6430542K11Rik) gene in murine species (Gene ID 108071). The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner.
Specificity
This antibody will not detect Isoform 2.
Immunogen
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of mouse Metabotropic Glutamate Receptor 5.
Application
Anti-Metabotropic Glutamate Receptor 5 Antibody, pain is an antibody against Metabotropic Glutamate Receptor 5 for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western Blotting Analysis: 0.1-0.5 μg/mL using ECL on rat brain microsomal preparation. Reacts with a band of ~132 kDa.
Immunohistochemistry: 0.1-0.2 μg/mL on rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in mouse hindbrain and rat colon tissue.
Immunohistochemistry: 0.1-0.2 μg/mL on rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in mouse hindbrain and rat colon tissue.
Quality
Evaluated by Western Blotting in microsomal preparation of rat brain tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Metabotropic Glutamate Receptor 5 in 10 µg of microsomal preparation of rat brain tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Metabotropic Glutamate Receptor 5 in 10 µg of microsomal preparation of rat brain tissue lysate.
Target description
~150 kDa observed. Uncharacterized band(s) may appear in some lysates.
Linkage
Replaces: AB1596; 06-451
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Brain tissue
Brain tissue
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Posttraumatic stress disorder-like induction elevates ?-amyloid levels, which directly activates corticotropin-releasing factor neurons to exacerbate stress responses.
Justice, NJ; Huang, L; Tian, JB; Cole, A; Pruski, M; Hunt, AJ; Flores, R; Zhu et al.
The Journal of Neuroscience null
Differential role for synaptojanin 1 in rod and cone photoreceptors.
Lars C Holzhausen,Alaron A Lewis,Kimberly K Cheong,Susan E Brockerhoff
The Journal of Comparative Neurology null
Mathieu Lafourcade et al.
PloS one, 2(8), e709-e709 (2007-08-09)
Cannabinoids have deleterious effects on prefrontal cortex (PFC)-mediated functions and multiple evidences link the endogenous cannabinoid (endocannabinoid) system, cannabis use and schizophrenia, a disease in which PFC functions are altered. Nonetheless, the molecular composition and the physiological functions of the
Assessment of the potential role of muscle spindle mechanoreceptor afferents in chronic muscle pain in the rat masseter muscle.
Lund, JP; Sadeghi, S; Athanassiadis, T; Caram Salas, N; Auclair, F; Thivierge et al.
Testing null
Effects of inflammation on the ultrastructural localization of spinal cord dorsal horn group I metabotropic glutamate receptors.
Mark H Pitcher, Alfredo Ribeiro-da-Silva, Terence J Coderre
The Journal of Comparative Neurology null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service