CMC0008
STEADY Chemically Competent Cells
for plasmid production using unstable DNA
About This Item
Recommended Products
biological source
Escherichia coli
grade
for molecular biology
form
buffered aqueous solution
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.
Genotype
recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Features and Benefits
- Clone unstable sequences with reliable results
- Available as chemically competent or electrocompetent cells
- Your choice of two packaging formats
- High efficiency: over 1 × 108 cfu/μg (chemi) or 1 × 1010 cfu/μg (electro)
- Excellent value
Components
- STEADY chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
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