G1N10
GenElute™ Mammalian Genomic DNA Miniprep Kits
sufficient for 10 purifications
Sinónimos:
Mammalian Genomic DNA Miniprep, Gen Elute
About This Item
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General description
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.
Application
- restriction endonuclease digestions
- PCR
- Southern blots
- sequencing reactions
- cloning
- ligation
Features and Benefits
- Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
- Elution volume: 200 - 400 μl
- Time required: 20 min after lysis
- A260/A280 ratio: 1.6 - 1.9
- Mechanical homogenization required: No
Principle
Other Notes
Legal Information
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
483.8 °F
flash_point_c
251 °C
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Artículos
GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Protocolos
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Contenido relacionado
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian cells and tissues. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
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