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Microbial conversion of L-ascorbic acid to L-erythroascorbic acid.

Bioscience, biotechnology, and biochemistry (2009-04-09)
Daiki Kyotani, Keiko Hasegawa, Hideo Ohishi, Wenhui Wu, Liyun Wang, Keiji Hasumi
ABSTRACT

We found that a strain of Penicillium sp. effectively converted L-ascorbic acid to a five-carbon analog, which was identified as L-erythroascorbic acid based on spectroscopic analysis. The conversion was achieved by growing culture or washed mycelia, with a yield of approximately 20-30% (mol/mol). The processes for the bioconversion and purification of the product are described.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(−)-Isoascorbic acid, 98%
Sigma-Aldrich
D-Isoascorbic acid, FG