822857

TSKgel^® SuperSW mAb HR Guard Column

phase diol, L × I.D. 4 cm × 6 mm, 4 μm particle size

• Synonym(s): TSKgel^® Size Exclusion (SW-Type) HPLC Guard Column
• UNSPSC Code: 41115711

Properties

• material: stainless steel
• Agency: suitable for USP L20; suitable for USP L59
• product line: TSKgel^®
• manufacturer/tradename: Tosoh SuperSW HR Guard, 22857
• parameter: ≤0.5 M salt concentration; 0-100% aqueous soluble organic solvents; 1 mL/min flow rate; 10-30 °C temperature
• technique(s): HPLC: suitable
• L × I.D.: 4 cm × 6 mm
• matrix: silica particle platform
• matrix active group: diol phase
• particle size: 4 μm
• pore size: 250 Å pore size
• operating pH: 2.5-7.5
• separation technique: size exclusion (SEC)

Description

General description

The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.

Physical form

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation