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Visiprep SPE Vacuum Manifold

DL (Disposable Liner), 24-port model

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About This Item

UNSPSC Code:
12000000

material

PTFE frit
polypropylene tube

Quality Level

product line

Visiprep

technique(s)

solid phase extraction (SPE): suitable

application(s)

food and beverages

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General description

The Visiprep DL (Disposable Liner) Vacuum Manifold eliminates the possibility of cross-contamination when processing a new sample on the same port. The liner consists of a PP female luer hub that attaches to the SPE, and thin-walled PTFE tubing that is threaded through the SPE port. This ensures that all SPE port and valve surfaces coming in contact with the sample can be replaced following each extraction.
SPE is a form of chromatography, and, as with other chromatographic techniques, control of flow rate is critical for maintaining reproducible extractions. Unlike other vacuum manifolds, the Visiprep system contains a patented valve system that allows for precise flow control through each SPE tube via rotating, independent, screw-type valves situated in each port within the manifold cover. Visiprep vacuum manifolds allow you to process up to 12 (12-port version) or 24 (24-port version) SPE samples simultaneously. Spacing between ports are slightly smaller on the 24-port versions than the 12-port versions so consideration of tube sizes is recommended.

Application

Visiprep SPE Vacuum Manifold was used to perform solid phase extraction to separate polyphenols from grapes, in order to determine the polyphenol content using LC-MS analysis. It was also used for concentration and purification of solvent extracts from surface water and sewage water sediments. Furthermore it was also used to extract and determine estrone in water using solid-phase extraction-enzyme-linked immunosorbent assay (SPE-ELISA).

Features and Benefits

Supplied with 100 disposable liners (Part No. 57059).
  • Patented screw-type valves within each SPE port for precise flow control
  • Glass basin will not dissolve, fog, or discolor when exposed to solvents
  • Legs on cover allow user to easily rest cover on work surface when removed from the manifold
  • Screw-type solvent-resistant vacuum bleed gauge and valve offer better sealing and vacuum control. Valve takes 1/4″ vacuum tubing.
  • PP collection vessel rack accommodates autosampler vials; small scintillation vials (22.75 mm O.D. recommended); 10 and 16 mm test tubes; and 1, 2, 5, and 10 mL volumetric flasks. An optional plate for 20 mL scintillation vials is available for 12-port models.

Legal Information

Visiprep is a trademark of Sigma-Aldrich Co. LLC

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Guangdi Wang et al.
Journal of environmental monitoring : JEM, 14(5), 1353-1364 (2012-03-23)
Endocrine disrupting compounds (EDCs), represented by steroid hormones, organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and bisphenol A have been determined in four sediment cores from the Gulf of Mexico, from New Orleans surface water (Lake Pontchartrain and Mississippi River), and
Angeles M Alonso Borbalán et al.
Journal of chromatography. A, 1012(1), 31-38 (2003-09-26)
There is considerable interest in the analysis of phenolic compounds due to their involvement in the organoleptic characteristics of foods and drinks, and their contribution to protection against cardiovascular diseases and cancers through their antioxidant activity. This article describes studies
Development of a solid-phase extraction-enzyme-linked immunosorbent assay method for the determination of estrone in water.
Li, Zhilan, et al.
Analytica Chimica Acta, 503, 171-177 (2004)

Articles

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Protocols

Polychlorinated biphenyls (PCBs) were once widely used in many industrial and commercial applications such as insulating fluids, dielectrics, and lubricants. This included their extensive use in electrical transformers. PCB use has been banned in many countries, and the Stockholm Convention currently prohibits their production and use.

Paprika is a spice made from dried sweet peppers, and is used for flavor and color in many types of cuisine. Contamination with polynuclear aromatic hydrocarbons (PAHs) can occur when pepper plants are exposed to these pollutants in the environment and/or during the drying process.

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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