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23348-U

Supelco

SP®-2560 Capillary GC Column

L × I.D. 75 m × 0.18 mm, df 0.14 μm

Synonym(s):

GC column, biscyanopropyl siloxane

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About This Item

UNSPSC Code:
12352211
eCl@ss:
32119290
NACRES:
SB.54

material

fused silica

Quality Level

100

Agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

321

df

0.14 μm

technique(s)

gas chromatography (GC): suitable (fast GC)

L × I.D.

75 m × 0.18 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Application

SP®-2560 capillary GC column may be used for analysing fatty acid methyl esters (FAME) using capillary gas liquid chromatography (GLC).

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
282715-02
279778-08
279778-05
279778-04
279778-03

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S Pegolo et al.
Journal of dairy science, 100(4), 2564-2576 (2017-02-13)
Buffalo milk is the world's second most widely produced milk, and increasing attention is being paid to its composition, particularly the fatty acid profile. The objectives of the present study were (1) to characterize the fatty acid composition of Mediterranean
M Cattani et al.
Journal of animal science and biotechnology, 8, 15-15 (2017-02-07)
In the last years, difficulties occurring in corn cultivation (i.e., groundwater shortages, mycotoxin contamination) have been forcing dairy farmers to consider alternative silages. Some experiments conducted on lactating cows have proven that the total replacement of corn silage with sorghum
Determination ofcis andtrans-Octadecenoic acids in margarines by gas liquid chromatography-infrared spectrophotometry.
Ratnayake, W. M. N., et al.
Journal of the American Oil Chemists' Society, 67, 804-810 (1990)
W M Nimal Ratnayake et al.
Journal of AOAC International, 85(5), 1112-1118 (2002-10-11)
This study examines the effect of the column operating temperature of 100 m SP-2560 and CP-Sil 88 capillary gas chromatographic (GC) columns on the separation of cis- and trans-octadecenoic (18:1) isomers in partially hydrogenated vegetable oils. The overlapping GC peaks
R R Gangidi et al.
Lipids, 39(6), 577-582 (2004-11-24)
Conjugated linoleic acid (CLA), an anticarcinogenic compound with numerous other health benefits, is present mainly in dairy and beef lipids. The main CLA isomer present in dairy and beef lipids is cis 9, trans 11 CLA at a 0.5% concentration.
View All Related Papers

Articles

New cis/trans FAME Standard for Optimising GC System Performance

AOCS and AOAC methods for determining the trans fatty acid composition of foods by gas chromatography require the use of a highly polar cyanosilicone capillary column to provide the best peak resolution attainable of the numerous geometric (cis & trans) and positional isomers.

Protocols

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Chromatograms

application for SPE, suitable for GC

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