WTA1

TransPlex^® Whole Transcriptome Amplification Kit

DNA polymerase separate.

• Synonym(s): Transcriptome Amplification Kit
• UNSPSC Code: 41121800
• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): whole genome amplification: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

TransPlex^®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Application

TransPlex^® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.

Suitable for use with downstream applications including:

• qPCR
• microarray analysis
• cloning

Features and Benefits

• Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
• Only 5 ng of starting material required to produce a highly representative library from total RNA
• Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Principle

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.

Legal Information

TransPlex is a registered trademark of Rubicon Genomics, Inc.

Kit Components Also Available Separately

• W4502 Water, Nuclease-Free Water, for Molecular Biology
• D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable