W0138
Western Blocker™ Solution
for HRP detection systems
Synonym(s):
Western blot blocking solution, blocking buffer
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41105329
NACRES:
NA.25
Recommended Products
![Western Blocking Reagent, Solution solution, sufficient for 10 blots (11921673001 [100 cm2]), sufficient for 60 blots (11921681001 [100 cm2])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Features and Benefits
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Lalit P Chandravanshi et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 34, 60-75 (2014-02-13)
In view of the increasing incidences of arsenic induced health effects and the vulnerability of the developing brain to its toxic effects, studies have been carried out to investigate the mechanism of arsenic induced cholinergic alterations and understand if such
A N Meleshko et al.
Experimental oncology, 35(1), 8-14 (2013-03-27)
Idiotype, the unique part of immunoglobulin molecule expressed on the surface of B-cells, represents a specific antigen for vaccination against lymphoma. We have developed a rapid method for immunoglobulin variable fragments cloning, assembling and expression of recombinant idiotype protein in
In vitro differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells.
Pournasr B, et al.
Archives of Iranian Medicine, 14, 244-249 (2011)
Ali Jahed et al.
Autonomic neuroscience : basic & clinical, 118(1-2), 32-42 (2005-03-30)
Post-ganglionic sympathetic neurons express the p75 neurotrophin receptor (p75NTR) and brain-derived neurotrophic factor (BDNF), which together have been implicated in controlling the degree of efferent innervation of peripheral organs [Kohn, J., Aloyz, R.S., Toma, J.G., Haak-Frendscho, M., Miller, F.D. 1999.
Ali Fathi et al.
Journal of proteomics, 101, 1-16 (2014-02-18)
Neural differentiation of human embryonic stem cells (hESCs) is a unique opportunity for in vitro analyses of neurogenesis in humans. Extrinsic cues through neural plate formation are well described in the hESCs although intracellular mechanisms underlying neural development are largely
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service