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Supelco

Starch (GO/P) Assay Kit

sufficient for 20 assays

Synonym(s):

Starch Measurement Kit

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About This Item

UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

100

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Related Categories

General description

Starch (GO/P) Assay Kit was used to determine the starch content, during an experimental study done in order to investigate the composition and structure of sorghum spent grains (SSG), which is a brewery co-product and is reported to have potential applications thereby adding value to the product. It was also used in starch extraction and quantification, during investigating the importance of OsGA2ox5 in GAs homeostasis, development, gravity responses and stress tolerance in rice. Furthermore, it also found application in being used in analysing factors affecting polyhydroxybutyrate accumulation , which are basically linear biodegradable polyesters produced by bacteria as a carbon store, in mesophyll cells of sugarcane and switchgrass.

Application

Starch (GO/P) Assay Kit was used to determine the starch content, during an experimental study done in order to investigate the composition and structure of sorghum spent grains (SSG), which is a brewery co-product and is reported to have potential applications thereby adding value to the product. It was also used in starch extraction and quantification, during investigating the importance of OsGA2ox5 in GAs homeostasis, development, gravity responses and stress tolerance in rice. Furthermore, it also found application in being used in analysing factors affecting polyhydroxybutyrate accumulation , which are basically linear biodegradable polyesters produced by bacteria as a carbon store, in mesophyll cells of sugarcane and switchgrass.
This kit is for the quantitative, enzymatic determination of starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by α-amylase and amyloglucosidase. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Suitability

Suitable for the quantitative, enzymatic determination of starch infood and other materials.

Principle

This kit is for the quantitative, enzymatic determination of starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by α-amylase and amyloglucosidase. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Kit Components Only

Product No.
Description
  • α-Amylase, Heat Stable .5 mL
  • Starch Assay Reagent 1 mL/vial
  • Glucose Oxidase/Peroxidase Reagent 1 capsule
  • o-Dianisidine Reagent 5 mg
  • Glucose Standard Solution .5 mL
  • Wheat Starch 1 g
  • Corn Starch 1 g

Signal Word

Danger

Hazard Statements

H302,H314,H334,H335,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Am. Assoc. Cereal Chem. Approved Methods, 76-12 (1995)
The composition and ultrastructure of sorghum spent grains.
Holmes CP
Journal of the Institute of Brewing, 119 (1-2), 41-47 (2013)
Zhiqian Lyu et al.
Asian-Australasian journal of animal sciences, 33(2), 286-293 (2019-09-05)
Two experiments were conducted to evaluate the effects of corn particle size and lipid form on the apparent total tract digestibility (ATTD) of energy and nutrients in diets for growing pigs. In Exp. 1, thirty barrows (initial body weight [BW]
J Mason Earles et al.
The New phytologist, 219(1), 89-97 (2018-04-18)
Rising temperatures and extended periods of drought compromise tree hydraulic and carbohydrate systems, threatening forest health globally. Despite winter's biological significance to many forests, the effects of warmer and dryer winters on tree hydraulic and carbohydrate status have largely been
Wenxuan Dong et al.
Asian-Australasian journal of animal sciences, 33(11), 1787-1796 (2020-02-15)
The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) of yellow dent corn sourced from different meteorological origins fed to growing pigs and develop equations to predict the DE and ME of yellow
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Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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