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SRP2107

Sigma-Aldrich

p53 (1-81), wild type, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

FLJ92943, LFS1, P53, TRP53

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~34.6 kDa

packaging

pkg of 10 μg

concentration

400 μg/mL

technique(s)

western blot: suitable

color

clear colorless

NCBI accession no.

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues.[1] It is a tetrameric nuclear DNA-binding phosphoprotein.[2] The gene encoding it is localized on human chromosome 17p13.1.[3][4]

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis.[5] It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein.[2] The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.[1][6]
p53 was identified as a tumor suppressor by showing that this protein has the ability to block transformation and to inhibit tumor cell growth. In addition, p53 is a transcription factor capable of regulating the expression of a subset of downstream genes. Mutation of two specific N-terminal residues in p53 (residues Leu22 and Trp23) impairs the ability of p53 to transactivate and has been correlated with its ability to bind TAFII40 and TAFII60 (or TAFII31 and TAFII70) suggesting that one or both of these interactions is important for activation. Mutation of residues 22 and 23 to Ala does not affect binding to TBP, although mutation of these residues to charged amino acids has been reported to disrupt the p53-TBP interaction. Different mutations in p53 gene have been characterized in a variety of human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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The Cell: A Molecular Approach (2000)
Chromosomal instability and tumors promoted by DNA hypomethylation.
Eden A, et al.
Science, 300(5618), 455-455 (2003)
The Cell: A Molecular Approach (2000)
S J Baker et al.
Science (New York, N.Y.), 249(4971), 912-915 (1990-08-24)
Mutations of the p53 gene occur commonly in colorectal carcinomas and the wild-type p53 allele is often concomitantly deleted. These findings suggest that the wild-type gene may act as a suppressor of colorectal carcinoma cell growth. To test this hypothesis
C A Finlay et al.
Cell, 57(7), 1083-1093 (1989-06-30)
DNA clones of the wild-type p53 proto-oncogene inhibit the ability of E1A plus ras or mutant p53 plus ras-activated oncogenes to transform primary rat embryo fibroblasts. The rare clones of transformed foci that result from E1A plus ras plus wild-type

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