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Key Documents

SAB4700151

Sigma-Aldrich

Monoclonal Anti-CD30-PE antibody produced in mouse

clone MEM-268, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Ki-1, Anti-TNFRSF8

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

phycoerythrin (R-PE) conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-268, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG

NCBI accession no.

NM_001243.3

UniProt accession no.

P28908

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TNFRSF8(943)

General description

The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin′s and Reed-Sternberg cells; it is also found in Burkitt′s lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).

Immunogen

Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Features and Benefits

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Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Igor Pavlov et al.
Clinical and vaccine immunology : CVI, 16(9), 1327-1331 (2009-07-17)
Testing for soluble CD30 (sCD30), an indicator of Th2 immune response, is a useful prognostic marker in solid organ transplantation, lymphoproliferative disorders, autoimmunity, and various parasitic diseases. In this study we report the development and validation of a fluorescent microsphere

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