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SAB4200781

Sigma-Aldrich

Anti-Haptoglobin antibody, Mouse monoclonal

clone HG-36, purified from hybridoma cell culture

Synonym(s):

Anti-HP, Anti-Zonulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HG-36, monoclonal

form

buffered aqueous solution

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

ELISA: 0.2-0.4 μg/mL using 10 μg/ml human haptoglobin for coating
dot blot: suitable
immunoblotting: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P00738

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HP(3240)

General description

Haptoglobin, also known as HP, is a serum α2-glycoprotein that exists as a tetramer, composed of two smaller identical alpha (α) chains and two larger identical beta (β) chains. The α-chains are linked to each other by a disulfide bond and each β-chain is similarly linked to an α-chain.
Monoclonal Anti-Haptoglobin specifically recognizes the three types of human haptoglobin (types 1-1, 2-1, and 2-2) The antibody recognizes both purified and haptoglobin in human serum, and shows no crossreactivity with human IgG, transferrin, C-reactive protein, α-1-acid glycoprotein, albumin, hemoglobin, or bovine, goat and sheep serum haptoglobin.

Immunogen

purified haptoglobin from pooled human plasma

Application

The antibody is recommended to use in various immunological techniques, including ELISA, dot blot, immunoprecipitation, and immunoblot . The antibody reacts against native and denatured (non-reduced) human haptoglobin but does not recognize the haptoglobin under reducing conditions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alessio Fasano
Physiological reviews, 91(1), 151-175 (2011-01-21)
The primary functions of the gastrointestinal tract have traditionally been perceived to be limited to the digestion and absorption of nutrients and to electrolytes and water homeostasis. A more attentive analysis of the anatomic and functional arrangement of the gastrointestinal
Kati Mokkala et al.
Metabolism: clinical and experimental, 69, 43-50 (2017-03-14)
Increased intestinal permeability with subsequent metabolic endotoxemia, i.e., elevated circulating levels of bacterial lipopolysaccharide, LPS, has been introduced as a novel initiator of obesity related metabolic disturbances in non-pregnant individuals. The objective was to investigate the extent to which intestinal
Haptoglobin: the evolutionary product of duplication, unequal crossing over, and point mutation.
B H Bowman et al.
Advances in human genetics, 12, 189-261 (1982-01-01)
S M Hossein Sadrzadeh et al.
American journal of clinical pathology, 121 Suppl, S97-104 (2004-08-10)
Haptoglobin is a positive acute phase protein that binds free hemoglobin and removes it from the circulation to prevent kidney injury, and iron lossfollowing hemolysis. Also, by bindingfree hemoglobin, haptoglobin functions as an antioxidant. In addition, haptoglobin acts as a
C J Chen et al.
Infection and immunity, 64(12), 5008-5014 (1996-12-01)
The majority of in vitro-grown Neisseria gonorrhoeae strains were unable to use hemoglobin as the sole source of iron for growth (Hgb-), but a minor population was able to do so (Hgb+). The ability of Hgb+ gonococci to utilize hemoglobin
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