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Key Documents

S3264

Sigma-Aldrich

Sodium phosphate dibasic

for molecular biology, ≥98.5% (titration)

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

Assay

≥98.5% (titration)

form

powder

pH

8.9-9.2 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 100 mg/mL

anion traces

chloride (Cl-): ≤40 ppm

cation traces

Fe: ≤20 ppm
heavy metals (as Pb): ≤10 ppm

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase and protease, none detected

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Application


  • Electrospraying of Bio-Based Chitosan Microcapsules Using Novel Mixed Cross-Linker: Experimental and Response Surface Methodology Optimization.: Published in Materials, this research explores the use of sodium phosphate dibasic as a cross-linker in the electrospraying of chitosan microcapsules. The study emphasizes the chemical′s importance in optimizing the microcapsule formation process for potential biomedical applications (Uko et al., 2022).

  • Compound treatment of thiolated citrus high-methoxyl pectin and sodium phosphate dibasic anhydrous improved gluten network structure.: This article from Food Chemistry demonstrates how sodium phosphate dibasic anhydrous enhances the gluten network structure when combined with thiolated citrus high-methoxyl pectin, suggesting applications in food science and nutrition (Chen et al., 2023).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andrew D Pucker et al.
Investigative ophthalmology & visual science, 51(6), 2949-2954 (2010-01-22)
The purpose of this work was to develop an enzymatic method of quantification of cholesterol and cholesterol esters derived from contact lenses, both in vitro and ex vivo. Lotrafilcon B (O2 Optix; CIBA Vision, Inc., Duluth, GA) and galyfilcon A
Menglin Liu et al.
Nature communications, 10(1), 2126-2126 (2019-05-11)
Repair of the endothelial cell barrier after inflammatory injury is essential for tissue fluid homeostasis and normalizing leukocyte transmigration. However, the mechanisms of endothelial regeneration remain poorly understood. Here we show that the endothelial and hematopoietic developmental transcription factor Sox17
Janelle M Spinazzola et al.
Human molecular genetics, 24(9), 2470-2481 (2015-01-22)
Loss of gamma-sarcoglycan (γ-SG) induces muscle degeneration and signaling defects in response to mechanical load, and its absence is common to both Duchenne and limb girdle muscular dystrophies. Growing evidence suggests that aberrant signaling contributes to the disease pathology; however
Kaisorn L Chaichana et al.
Journal of neuroscience methods, 180(1), 116-125 (2009-05-12)
The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such
Megan A Rees et al.
Journal of proteome research, 14(1), 120-132 (2014-10-21)
Interactions between a host and a bacterial pathogen are mediated by cross-talk between molecules present on, or secreted by, pathogens and host binding-molecules. Identifying proteins involved at this interface would provide substantial insights into this interaction. Although numerous studies have

Protocols

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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