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P5975

Millipore

Amino-terminal Met-FLAG-BAP™ Fusion Protein

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About This Item

MDL number:
MFCD01632954
UNSPSC Code:
12352202
NACRES:
NA.32

mol wt

~49 kDa

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

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Application

Amino-terminal Met-FLAG-BAP™ Fusion Protein is useful for assurance of the functional integrity of ANTI-FLAGÒ M2 and M5 monoclonal antibodies in immunological procedures such as Western blotting, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy and FACS.
Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Amino-terminal Met-FLAG-BAP™ control protein is a 468 amino acid N-terminal Met-FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular weight of 49.4 kDa. The Amino-terminal Met-FLAG-BAP control protein migrates as a 45-55 kDa band by SDS-PAGE depending on the electrophoretic conditions.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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