Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

P4957

Sigma-Aldrich

Poly-ʟ-Ornithine

synthetic, mol wt 30,000-70,000, liquid, 0.01%, suitable for cell culture

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
27378-49-0
MDL number:
MFCD00286305
UNSPSC Code:
12352202
PubChem Substance ID:
24898574
NACRES:
NA.75

product name

Poly-L-ornithine solution, mol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

biological source

synthetic

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

30,000-70,000

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

SMILES string

Br.NCCCC(N)C(O)=O

InChI

1S/C5H12N2O2.BrH/c6-3-1-2-4(7)5(8)9;/h4H,1-3,6-7H2,(H,8,9);1H

InChI key

GWRQMKDBBHFVIZ-UHFFFAOYSA-N

General description

Poly-L-ornithine is a positively charged synthetic amino acid polymer.

Application

Poly-L-ornithine solution has been used in the induction of differentiation.
Poly-L-ornithine has been used successfully for the attachement and study of neural cell differentiation and outgrowth. Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2.

Biochem/physiol Actions

Poly-L-ornithine helps to improve the penetration of substances into cells. It can induce the uptake of DNA.

Preparation Note

Prepared in cell culture grade water.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Plant Protoplasts: International Review of Cytology (2013)
Interferon (2009)
Ewoud B Compeer et al.
Nature communications, 9(1), 1597-1597 (2018-04-25)
Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that
Differential effect of activin on mouse embryonic stem cell differentiation in insulin?secreting cells under nestin?positive selection and spontaneous differentiation protocols.
Jafary H, et al.
Cell Biology International, 32(2), 278-286 (2008)
Areechun Sotthibundhu et al.
EXCLI journal, 15, 829-841 (2017-03-10)
Melatonin, secreted mainly by the pineal gland, plays roles in various physiological functions including protecting cell death. We showed in previous study that the proliferation and differentiation of precursor cells from the adult mouse subventricular zone (SVZ) can be modulated
View All Related Papers

Articles

Poly-lysine | Poly-D-Lysine | Poly-L-Lysine

Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

See All

Protocols

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service