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P4630

Sigma-Aldrich

Protease from bovine pancreas

Type I, ≥5 units/mg solid

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About This Item

CAS Number:
9001-92-7
EC Number:
232-642-4
MDL number:
MFCD00132092
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

bovine pancreas

Quality Level

200

type

Type I

form

powder

specific activity

≥5 units/mg solid

storage temp.

−20°C

Application

Non-specific Protease Activity Assay Video
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Product P4630 is from bovine pancreas. Protease from bovine pancrease (type I) has been used for the extraction of hemicellulose.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Quality

Crude

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Precautionary Statements

P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P A Hedin et al.
The Journal of nutrition, 124(12), 2458-2465 (1994-12-01)
The southwestern corn borer, Diatraea grandiosella Dyar, is a major pest of corn, Zea mays L., in the southern United States. To search for plant allelochemicals, chemically defined insect diets are commonly used, but southwestern corn borer larvae did not
Werend Boesmans et al.
Glia, 67(6), 1167-1178 (2019-02-08)
Coordination of gastrointestinal function relies on joint efforts of enteric neurons and glia, whose crosstalk is vital for the integration of their activity. To investigate the signaling mechanisms and to delineate the spatial aspects of enteric neuron-to-glia communication within enteric
Marc Machnik et al.
Drug testing and analysis, 9(9), 1372-1384 (2016-09-24)
Methylxanthines positives in competition samples have challenged doping control laboratories and racing jurisdictions since methylxanthines are naturally occurring prohibited substances and often constituents of feed. For theobromine, an international threshold (renamed in International Residue Limit, IRL) of 2 µg/mL in urine
Nir Qvit et al.
Pharmaceuticals (Basel, Switzerland), 15(3) (2022-03-27)
Myocardial infarction is the leading cause of cardiovascular mortality, with myocardial injury occurring during ischemia and subsequent reperfusion (IR). We previously showed that the inhibition of protein kinase C delta (δPKC) with a pan-inhibitor (δV1-1) mitigates myocardial injury and improves
U Klöckner
Pflugers Archiv : European journal of physiology, 424(3-4), 231-237 (1993-08-01)
Calcium-activated chloride currents were studied by the patch-clamp technique in vascular smooth muscle cells (VSMC) isolated from human mesenteric arteries. Bath application of 20 mM caffeine caused the cell membrane to depolarize by a calcium-activated inward current that peaked to
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Protocols

Universal Protease Activity Assay: Casein as a Substrate

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases, which is what we do during our quality control procedures.

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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