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P1526

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, solid

Synonym(s):

OPD

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein:
3912045
EC Number:
210-418-7
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898213
NACRES:
NA.83

product name

o-Phenylenediamine dihydrochloride, peroxidase substrate

Quality Level

300

form

solid

color

white to off-white

mp

258 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.

Caution

May darken in storage.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Malaria journal, 10, 206-206 (2011-07-29)
The Anopheles gambiae gSG6 is an anopheline-specific salivary protein which helps female mosquitoes to efficiently feed on blood. Besides its role in haematophagy, gSG6 is immunogenic and elicits in exposed individuals an IgG response, which may be used as indicator
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Analytical and bioanalytical chemistry, 402(5), 1827-1846 (2011-09-29)
This review highlights the importance of coupling molecular imprinting technology with methodology based on electrochemical techniques for the development of advanced sensing devices. In recent years, growing interest in molecularly imprinted polymers (MIPs) in the preparation of recognition elements has
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Bioresource technology, 101(24), 9415-9420 (2010-08-17)
Nanoporous copper (NPC) with a pore size of 100-200 nm was prepared by simply dealloying Al(60)Cu(40) alloy in a 5 wt.% HCl solution. The NPC was characterized by scanning electron microscopy and nitrogen adsorption techniques. Horseradish peroxidase (HRP) was immobilized
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