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NA2010

Sigma-Aldrich

GenElute™ Blood Genomic DNA Kit

sufficient for 70 purifications

Synonym(s):

Blood Genomic DNA, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

usage

sufficient for 70 purifications

Quality Level

200

technique(s)

DNA purification: suitable

storage temp.

15-25°C
room temp

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General description

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

GenElute™ Blood Genomic DNA Kit has been used:
  • to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits
  • to isolate DNA from the blood samples 
  • to extract whole blood DNA from the left blood cells

The purified genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning

Features and Benefits

  • Starting material: Up to 200 μl of fresh or aged blood
  • Expected yield: Up to 10 μg
  • Elution volume: 400 μl
  • Time required: <40 min
  • A260/A280 ratio: 1.6 - 1.9
  • Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate

Principle

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

recommended

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Description
Pricing

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P2308

Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

D9780

Lambda DNA Hind III Digest, for DNA electrophoresis

R6148

RNase A solution

NA2020

GenElute™ Blood Genomic DNA Kit, sufficient for 350 purifications

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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Protocols

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

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