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Key Documents

N4762

Sigma-Aldrich

Newborn Calf Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonym(s):

NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera

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About This Item

MDL number:
MFCD00163621
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine (calf)

Quality Level

500

sterility

sterile-filtered

form

liquid

composition

hemoglobin, ≤20 mg/mL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤100 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

NCS is useful in a wide range of cell culture and organ preservation applications. For example, it is used in cornea preservation and cryopreservation of bovine embryos. NCS is used in cell culture to support the maturation of oocytes; to study the function of tubular epithelial cells; and to develop primary cultures of cells such as visceral adipocytes.

Preparation Note

Collected from calves that are 10 days old or less.
Prepared by heating at 56°C for 30 minutes.

Analysis Note

Endotoxin tested

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Ümüt Cirit et al.
Theriogenology, 128, 218-224 (2019-02-21)
The present study evaluated follicular superstimulatory (FSS) and superovulatory (SOV) responses and in vivo embryo production in lactating Simmental cows treated with FSH starting 1 or 2 days after follicle aspiration (FA). The performance of a lengthened superovulation program, named 6dFSH-P36-hCG60
Mette Hemmingsen et al.
PloS one, 8(5), e63638-e63638 (2013-06-01)
High cell density is known to enhance adipogenic differentiation of mesenchymal stem cells, suggesting secretion of signaling factors or cell-contact-mediated signaling. By employing microfluidic biochip technology, we have been able to separate these two processes and study the secretion pathways.
Adela Ngwewondo et al.
PLoS neglected tropical diseases, 12(6), e0006565-e0006565 (2018-06-14)
Ivermectin is the only drug currently recommended for the treatment of onchocerciasis, the second leading infectious cause of blindness in the world. This drug kills only the first stage larvae-microfilariae (mf) of Onchocerca volvulus and is to be used cautiously
Adela Ngwewondo et al.
Experimental parasitology, 198, 71-78 (2019-02-17)
Despite the efforts employed for the control of onchocerciasis, the latter has remained a significant public health problem, due mainly to the lack of safe and effective adult worm drugs and/or microfilaricides that do not kill Loa loa microfilariae (mf).
Kyoko Shimizu et al.
Cell biology international, 30(4), 381-388 (2006-03-15)
We have recently developed a primary culture system for visceral adipocytes (VAs) using stomal-vascular cells (SVCs) isolated from the mesenteric fat tissue of male Sprague-Dawley rats of 3-5 weeks of age. Modified Dulbecco's modified Eagle medium (DMEM)/F12 containing 17 microM
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