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N3790

Sigma-Aldrich

Nutrient Mixture F-12 Ham

StableCell™, with stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Nutrient Mixture F-12 Ham, Ham’s F-12

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

product name

StableCell™ Ham′s F-12, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

HEPES: no
sodium pyruvate: 0.11 g/L
stable glutamine: yes
phenol red: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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Application

Developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells.
This Nutrient Mixture F-12 Hams differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fumitaka Kawakami et al.
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Henri J Huttunen et al.
Journal of molecular neuroscience : MN, 37(1), 6-15 (2008-07-12)
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