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Sigma-Aldrich

Keratinocyte Growth Factor human

HumanKine®, >95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonym(s):

FGF-7, KGF

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About This Item

CAS Number:
148348-15-6
MDL number:
MFCD00212245
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Keratinocyte Growth Factor human, KGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

4-20 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 17-30 kDa (glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

P21781

storage temp.

−20°C

Gene Information

human ... FGF7(2252)

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General description

Keratinocyte Growth Factor (KGF) belongs to the fibroblast growth factor 7 (FGF-7) family. It is produced by the mesenchymal cells, γδ T cells, and fibroblasts in several organs. The KGF gene is located on the human chromosome at 15q21.2.

Application

Keratinocyte Growth Factor (KGF) human has been used as a component of the keratinocyte differentiation medium.

Biochem/physiol Actions

Keratinocyte Growth Factor (KGF) exerts paracrine effects on mesenchymal cells. It exhibits cryoprotective effects on epithelial cells of the lungs, gastrointestinal tract, and the lungs. KGF participates in the activation of IIIb isoform of the fibroblast growth factor (FGF)-receptor 2 (FGFR2-IIIb) present on keratinocytes. This activation further favors proliferation, migration, and survival of keratinocytes.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael J LaFemina et al.
American journal of physiology. Lung cellular and molecular physiology, 299(6), L724-L734 (2010-09-14)
Keratinocyte growth factor (KGF) has efficacy in several experimental models of lung injury; however, the mechanisms underlying KGF's protective effect remain incompletely understood. This study was undertaken to determine whether KGF augments barrier function in primary rat alveolar epithelial cells
Laxmi Yeruva et al.
mBio, 5(3), e01241-e01214 (2014-06-26)
It is not currently possible to predict the probability of whether a woman with a chlamydial genital infection will develop pelvic inflammatory disease (PID). To determine if specific biomarkers may be associated with distinct chlamydial pathotypes, we utilized two Chlamydia
Shuichi Tobinaga et al.
Acta histochemica et cytochemica, 48(3), 83-94 (2015-07-15)
Pulmonary emphysema is a progressive disease with airspace destruction and an effective therapy is needed. Keratinocyte growth factor (KGF) promotes pulmonary epithelial proliferation and has the potential to induce lung regeneration. The aim of this study was to determine the
Takeshi Kobayashi et al.
Experimental eye research, 135, 109-117 (2015-02-17)
Interactions between stromal and epithelial cells play important roles in the development, homeostasis, and pathological conditions of the cornea. Soluble cytokines are critical factors in stromal-epithelial interactions, and growth factors secreted from corneal stromal cells contribute to the regulation of

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