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Merck

H6269

Sigma-Aldrich

Hexadecyltrimethylammonium bromide

for molecular biology, ≥99%

Synonym(s):

CTAB, Cetrimonium bromide, Cetyltrimethylammonium bromide, Palmityltrimethylammonium bromide

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About This Item

Linear Formula:
CH3(CH2)15N(Br)(CH3)3
CAS Number:
Molecular Weight:
364.45
Beilstein:
3598189
EC Number:
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NA.31

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grade

for molecular biology

Quality Level

description

cationic

Assay

≥99%

form

powder or crystals

mol wt

micellar avg mol wt 62,000

aggregation number

170

pH

5.0-7 (25 °C, 36.4 g/L)

CMC

0.92
1 mM (20-25°C)

mp

248-251 °C (lit.)

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Application

Hexadecyltrimethylammonium bromide has been used:

  • as a component of DNA extraction buffer[1] and RNA extraction buffer[2]
  • as a constituent of dye dissolution buffer[3]

Biochem/physiol Actions

Hexadecyltrimethylammonium bromide also called cetyltrimethylammonium bromide (CTAB), is a cationic surfactant.[4] CTAB method is an efficient, cost and time effective method of DNA extraction.[5][6]

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral - STOT SE 3

Target Organs

Gastrointestinal tract, Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

471.2 °F - closed cup

Flash Point(C)

244 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Investigation of the Orientation of C T A
Karaca S, et al.
Journal of Chemistry, 2013 (2012)
The importance of the CTAB surfactant on the colloidal seed-mediated synthesis of gold nanorods
Smith DK and Korgel BA
Langmuir, 24(3), 644-649 (2008)
Two unrelated double-stranded RNA molecule patterns in Gremmeniella abietina type A code for putative viruses of the families Totiviridae and Partitiviridae
Tuomivirta TT and Hantula J
Archives of Virology, 148(12), 2293-2305 (2003)
Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes
Bomer M, et al.
Bio-protocol, 8(1), 11-17 (2018)
Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC
Pandey SS, et al.
Archives of Virology, 7, e2410-e2410 (2017)

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