GL0010
Golgi Isolation Kit
sufficient for 50 g (tissue)
Synonym(s):
Golgi Kit, Isolation Kit for Golgi
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
usage
sufficient for 50 g (tissue)
Quality Level
technique(s)
fractionation: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Application
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Analysis Note
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
Kit Components Also Available Separately
Product No.
Description
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDS
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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Articles
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.
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