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GE80-6483-56

2-D Quant Kit

Cytiva 80-6483-56

Synonym(s):

2D Quantification Kit, Protein Quant Kit

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

feature

wetted part: no

manufacturer/tradename

Cytiva 80-6483-56

packaging

pkg of 500 assays

storage temp.

2-8°C

Related Categories

General description

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

Common spectrophotometric methods of quantitating protein rely either on Coomassie dye binding or protein-catalyzed reduction of cupric (Cu2+) ion to cuprous (Cu+) ion. Dye-binding assays cannot be used in the presence of any reagent that also binds the dye. This includes carrier ampholytes and detergents such as PlusOne CHAPS, SDS, and Triton X-100. Assays that depend on the reduction of cupric ion cannot be used in the presence of reductants such as DTT, or in the presence of reagents that form complexes with cupric ion, such as thiourea or EDTA.

The 2-D Quant Kit procedure works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein. Precipitated proteins are resuspended in a copper-containing solution and unbound copper is measured with a colorimetric agent. The absorbance at 480 nm is inversely related to the protein concentration. The assay has a linear response to protein in the range of 0 to 50 μg and recommended sample volume is 1 to 50 μ.

Features and Benefits

  • Accurately determine protein concentration in the presence of 2% SDS, 1% DTT, 8 M urea, 2 M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer.
  • Quantitatively precipitates proteins while leaving interfering substances behind.
  • Linear response in the range of 0 to 50 μg protein, with recommended sample volumes of 1 to 50 μ.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Pictograms

Environment
GHS09

Signal Word

Danger

Hazard Statements

H314,H335,H411

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Milan O Urban et al.
Frontiers in plant science, 12, 628167-628167 (2021-06-29)
The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V)

Protocols

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

Desalting Samples Using Mini Dialysis Kit

Mini Dialysis Kit from Cytiva is designed for the dialysis of small sample volumes with minimal handling and sample loss and reduces the pronounced streaking on 2-D gels caused by low-molecular-weight contaminants.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Western Blotting Sample Preparation

The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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