GE29-0005-94

His GraviTrap^™ TALON^®

Cytiva 29-0005-94, pack of 10 columns

• Synonym(s): His GraviTrap
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: TALON^® Superflow Cobalt-IMAC
• packaging: pack of 10 columns
• manufacturer/tradename: Cytiva 29-0005-94
• bed volume: 1 mL
• column I.D.: 15 mm
• matrix: 6% cross-linked agarose
• particle size: 60-160 μm
• cleaning: 2-14(Co²⁺-stripped medium)
• working range: 3-12(Co2+-stripped medium)
• capacity: ≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary); ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
• storage temp.: 2-8°C

Description

General description

His GraviTrap^™ Talon^® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.

Application

His GraviTrap^™ Talon^® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap^™ Talon^® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon^® Superflow medium is negligible.

Features and Benefits

• Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
• Ready-to-use gravity flow column prepacked with 1 mL Talon^® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• One run takes about 30 minutes, and large sample volumes can be applied all at once.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva

Talon is a registered trademark of Clontech

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids