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Key Documents

GE28-9365-44

HiPrep Sp Fast Flow 16/10

Cytiva 28-9365-44

Synonym(s):

Fast Flow Column, Fast Flow SP Column, HiPrep SP Column

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About This Item

UNSPSC Code:
47101511
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9365-44

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiPrep SP FF is a strong cation exchanger prepacked with SP Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

HiPrep SP FF are prepacked, ready to use columns for ion exchange chromatography. They provide fast, preparative separations of proteins and other biomolecules.

Features and Benefits

  • HiPrep columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • Good flow rates and high capacity.
  • The industry standard for ion exchange chromatography.
  • Compatible with single-pump configurations and chromatography systems such as AKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to perform a purification of His-tagged membrane proteins.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

See All

Protocols

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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