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GE28-9180-07

PD Minitrap G-25

Cytiva 28-9180-07

Synonym(s):

Protein purification column

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About This Item

UNSPSC Code:
41115709
NACRES:
NA.56

feature

wetted part: no

packaging

pkg of 50 ea (columns)

manufacturer/tradename

Cytiva 28-9180-07

storage condition

dry media

bed size

9.7 mm × 28 mm

bed volume

2.1 mL

column I.D.

9.7 mm

matrix

cross-linked dextran

particle size

>50 μm (dry)
85-260 μm

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

General description

PD MiniTrap G-25 is a prepacked, single-use column for cleanup of large biomolecules (such as proteins >5000 Mr) by removal of buffer salts and low molecular weight compounds.

Application

Protein sample preparation. Desalting/Buffer Exchange/Cleanup.

Features and Benefits

  • Customizable protocols to use gravity flow or centrifugation.
  • Easy-to-use method for desalting and buffer exchange of sample volumes of 0.1 mL to 0.5 mL for gravity and 0.2 mL to 0.5 mL with centrifugation
  • Alternative to dialysis for protein desalting, thus saving time and reducing labor costs
  • Packed with Sephadex G-25 Medium resin

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Legal Information

MiniTrap is a trademark of Cytiva

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra products are magnetic beads designed for high capacity small-scale purification/screening of monoclonal and polyclonal antibodies from various species.

The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service