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GE17-6000-87

IPG-Buffer, pH 3-10

Cytiva 17-6000-87

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

sterility

non-sterile

form

liquid

feature

wetted part: no

packaging

pkg of 1 mL

manufacturer/tradename

Cytiva 17-6000-87

color

Colorless to light yellow

pH range

3-10

solubility

cold water: soluble (Soluble in cold water and hot water)

storage temp.

2-8°C

General description

IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline® DryStrip gels.

Application

Each IPG Buffer type produces more uniform conductivity along the Immobiline® DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining.

IPG buffers are supplied in 1-ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.

Features and Benefits

  • For use with Immobiline® DryStrip gels to improve Protein solubility.
  • Using Immobiline® DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting Low abundance Proteins.
  • Improved IPG buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional Protein spots in high molecular weight and basic pH areas.
  • Eliminates background staining.
  • Ensures optimal conditions for IEF separation.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Immobiline is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Troubleshooting Second-Dimension SDS-PAGE: Multiphor™ II Electrophoresis

This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.

Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit

This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.

Protocols

Protease Inhibition Using Protease Inhibitor Mix

Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva Healthcare is designed to disrupt cell or tissue samples for protein extraction

Using the Strip Holder for Gel Rehydration

This page shows how to use the IPGphor fixed-length Strip Holders from Cytiva for gel rehydration in 2-D electrophoresis.

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